Amal Kanti Paul
interest in this laboratory concentrates on the physiology
and biochemistry of synthesis and accumulation of biopolyesters,
polyhydroxyalkanoates (PHAs) by diverse heterotrophic and
autotrophic bacteria isolated from natural environments.
Efforts are being made to develop cost effective method
for production of novel PHAs with improved properties from
renewable resources and to link PHA synthesis with bacterial
metabolism and differentiation.
is a strong interest in studying intracellular utilization
and mobilization of accumulated PHAs by intracellular PHA
depolymerise (i-PHA depolymerise) as well as in vitro degradation
of homo- and copolymers of short-chain-length hydroxyalkanoic
acids (scl-PHAs) with emphasis on isolation and characterization
of extracellular PHA depolymerises (e-PHA depolymarase).
Studies are also conducted on microbial ecology of serpentine
soils, a naturally occurring metal-percolated ecosystem
inhabited by endemic nickel hyperaccumulators. Diversity,
metabolic physiology and metal resistance in serpentine
and non-serpentine microflora are compared. Metal-microbe
interactions in rhizo-microflora of metal hyperaccumulating
plants are also studied for application in bioremediation
Microorganisms in chromite mining biotopes are another new
field of research for exploring their potentials in metal
prospecting and bioremediation. Bioleaching of metals from
mine overburden and biosorption of metals help in removal
and recovery of toxic metals. Chromium-resistant bacteria
ubiquitous in chromite mine environment show bioreduction
and bioaccumulation potential. Search for chromium reductases
in bacteria from anthropogenic and geogenous sources are
Taxonomy, Biodiversity and Conservation, Biosystematics,
Palynology, Ethnobotany, Medicinal Plants, and Seed and
enhancement in relation to secondary metabolites and metabolomics.
elicitation of secondary metabolite accumulation in plants.
diversity and chromosome constitution as associated with
difference in chemical constituents in plants
vitro propagation and conservation of recalcitrant species
Swapan Kumar Datta
Molecular Biology, Biotechnology, genomics and Translational
Research and International Agriculture policy.Single-cell
development and differentiation in plants (microspore- and
protoplast-derived embryogenesis and artificial seed development
in cereals; in vitro laticifer differentiation; cytodifferentiation
and organ induced chemodifferentiation in plants).
and demonstrated stable transgene expression using protoplast,
Agrobacterium and Biolistic transformation driven by tissue-specific
and constitutive-promoters in rice.
the genetically engineered homozygous indica rice
developed from haploid embrogenic cell suspension culture
and field-evaluate the hybrid Bt rice, characterize sd1gene,
and pyramid transgenes (Bt, Xa21 and PR-Protein genes) and
define their function in rice.
and demonstrated the marker free elite transgenic indica
cultivars with biological and molecular characterization.
"Golden" indica rice with genes for beta-carotene (pro-vitaminA)
and ferritin gene (iron) for nutrititious rice.
participating in developing global vision of plant biotechnology
and relevant use in India.
analysis and metabolomics
of important plant secondary metabolites
of plant extracts and constituents
protocols/diagonistic kits for assessing genotoxicity of
chemicals disposed through natural and anthropogenically
of flyash by Vetiver plant
and anticlastogenic effects of some plants of ethnobotanical
of cytogenetic damage and DNA damage in vitro in human lymphocytes
induced by arsenic and fluoride through drinking water
of plants in the Indian sub continent since last 250 M.Y
- Palynostratigraphy, Ecostratigraphy
of Palaeoshoreline and hydrocarbon exploration
Archaeobotany, Entomopalynology, Melittopalynology, Forensic
- Animal Interaction and their co-evolution
analysis and Palaeophytochemistry
Comparative analysis of different in vitro plant regeneration
systems for efficient clonal propagation of different medicinal
plants and studies of both physical and chemicals factors
responsible for organogenic and embryogenic responses during
regeneration. Effects of in vitro environment on chromosome
status, total protein profile and isozyme activities of
the regenerates during organogenic and embryogenic responses.
In vitro propagation of different medicinal plants using
different systems to standardize efficient, simple and cost
effective protocol for conservation and improvement of medicinal
principle contents by manipulating culture regimes and by
addition of precursor molecules/elicitors during growth
in culture to identify elite clones. Also, hairy root induction
following transformation with Agrobacterium rhizogenes and
subsequent root culture are also being carried out to compare
the active principle contents of pharmacological importance
of these plants with the values of normal culture methods
adopted for improvement. The clones regenerated through
different systems are compared with their mother plants
with respect to their chromosomal status, total protein
pattern and certain isozyme activities.
Studies on genome diversity of different medicinal plants
utilizing cytological, cytochemical and molecular approaches.
The effects of such genome diversity on in vitro responses
during growth and development of these plants in culture
are also being analyzed
Asok Kumar Biswas
Physiology and Plant Biochemistry
oxide as signalling molecule in SAR and ISR:
of defense related NOS with resistance and susceptibility
of plant. Role of nitric oxide in the signalling network
of SAR and ISR.
Development & Nutracetical Research:
of antimicrobial, hepatoprotective, antidiabetic, cardioprotective,
anticancer drugs from wild mushrooms.
Control of Phytopathogens:
work focussed on isolation of potential antagonist having
a wide range of defense weapon and development of a cost
effective media for large scale production and formulation.
polymorphism and taxonomic infrastructure of wild and cultivated
mushrooms as determined by RAPD analyses, isozyme profiles,
characterization of plasmid and ecomorphological characters.
Research Interest is mainly focused on Algal Biotechnology
in relation to Bioremediation and Aquaculture, Phytoplankton
Dynamics and Ecological modeling and Microalgal diversity
of Kolkata and surroundings with special reference to Genomic
diversity and Molecular phylogeny of cyanobacteria.
study includes removal of heavy and toxic metals and radio-nuclides
using Algae as bio-reagents from natural habitat and in
vitro system using 'Tracer packet Technique', 'Neutron Activation
methods' and 'Atomic Absorption Spectrophotometry'. Separation
of precious metal gold and its bio- transformation to nano-gold
has already been reported by our group. To understand Algae-metal
interaction at cellular level, mode of action of individual
metal like Pb, Cr,Cd, Au and As for particular algal genus
is also determined. Different models of Bio-filter of toxic
metals have been developed using algae as bioreagent. Work
is going on the algal flora of Arsenic contaminated villages
of North 24-parganas to explore arsenic tolerant algal genera
and to develop bio-filter. To identify arsenic tolerant
gene from cyanobacteria is also included in the study.
of highly nutritious, non-conventional local algal genera
in aquaculture feed preparation and their role in fish dietetics
in relation to growth and immunity is other program of our
work. Different types of fish feed like, 'Total Supplement'
& 'Value Added Feed' have been found suitable for early
larval transformation of prawn. Work is going on for determining
the nutraceutical properties of value added algal feed for
carp fishes and colored fishes. Mass production of economically
useful microalgae using Photobioreactor and Open race way
Pond and Regular training of the villagers for mass cultivation
and feed production is also a part our Research Program.
is going on development of ecological model based on phytoplankton
diversity in relation to seasonal variation, nutrient cycle
and other physico- chemical parameters of Fresh water wetland,
Estuary and Coastal area of West Bengal .
Dr. Binay Chaubey (On lien)
C Virus (HCV) is a major cause of chronic active hepatitis,
hepatocellular carcinoma and liver cirrhosis. There is no
vaccine available against this virus and the current standard
therapy for HCV entails a concoction of pegylated interferon
alfa (PEG-IFN a) and ribavirin (RBV), both of which are
non-specific antiviral drugs. Although this therapy has
shown some promising results, it is fraught with limited
efficacy, resistance problems, poor tolerability and high
cost of manufacture, underscoring the need for new and more
effective therapy. The present focus of research is to inhibit
the viral replication using siRNA. This is highly versatile,
simple, straightforward and cost effective approach, which
is able to significantly down regulate, the target genes
in a sequence specific manner. This is a promising approach,
which can overcome the limitations associated with the existing
therapy. The main strategy in this study will be to deliver
multiple short hairpin RNAs (shRNA) targeted against both
the host cell factors and the conserved domains of viral
5' and 3' untranslated regions (UTR) and nonstructural genes
through self inactivating (SIN) lentiviral vectors. The
SIN lentiviral vectors unlike other vectors (adeno and retro
virals) can effectively integrate even in non-dividing host
cells and manifest lasting RNAi effect.
the HIV infected people mitochondrial dysfunction in hepatocytes
and other HIV infected cells is a leading cause of cellular
death. Several viral proteins such as HIV-1 Vpr and Gag
interact with and alter the organization of the mitochondrial
membrane. This may impair oxidative phosphorylation and
electron transport mechanisms resulting in depletion of
cellular ATP pool and accumulation of reactive oxygen species
(ROS). Interestingly, presence of a high copy number of
HIV-1 RNA has been reported in the mitochondrial compartment.
The rationale for this accumulation is not well understood.
Another pertinent question is the origin of HIV-1 RNA with
in the mitochondria. These questions need to be addressed
meticulously and carefully so that drugs directed to the
mitochondria can be designed in order to contain the viral
propagation in the mitochondria as well as sustain the mitochondrial
integrity. The results expected from these studies will
provide a new insight in our understanding of mitochondrial
dysfunction associated with HIV-1 infection and antiretroviral
treatment. This may significantly influence the ongoing
treatment decisions of AIDS patients and aid in better drug
designing and consequently improved management and control
of AIDS patients.
cytogenetics; Genetic transformation and metabolic engineering
biology; Plant Molecular Biology
mechanism of circadian rhythm in Neurospora.
fingerprinting of edible mushroom of West Bengal and making
a data base.
of wild edible mushrooms