Binay Chaubey

Associate Professor

Email
bchaubey@hotmail.com
Mobile
+91 943 209 5551


Research Interest

Hepatitis Research: Hepatitis C Virus (HCV) is a major cause of chronic active hepatitis, hepatocellular carcinoma and liver cirrhosis. There is no vaccine available against this virus and the current standard therapy for HCV entails a concoction of pegylated interferon alfa (PEG-IFN a) and ribavirin (RBV), both of which are non-specific antiviral drugs. Although this therapy has shown some promising results, it is fraught with limited efficacy, resistance problems, poor tolerability and high cost of manufacture, underscoring the need for new and more effective therapy. The present focus of research is to inhibit the viral replication using siRNA. This is highly versatile, simple, straightforward and cost effective approach, which is able to significantly down regulate, the target genes in a sequence specific manner. This is a promising approach, which can overcome the limitations associated with the existing therapy. The main strategy in this study will be to deliver multiple short hairpin RNAs (shRNA) targeted against both the host cell factors and the conserved domains of viral 5 and 3 untranslated regions (UTR) and nonstructural genes through self inactivating (SIN) lentiviral vectors. The SIN lentiviral vectors unlike other vectors (adeno and retro virals) can effectively integrate even in non-dividing host cells and manifest lasting RNAi effect.

HIV Research: Among the HIV infected people mitochondrial dysfunction in hepatocytes and other HIV infected cells is a leading cause of cellular death. Several viral proteins such as HIV-1 Vpr and Gag interact with and alter the organization of the mitochondrial membrane. This may impair oxidative phosphorylation and electron transport mechanisms resulting in depletion of cellular ATP pool and accumulation of reactive oxygen species (ROS). Interestingly, presence of a high copy number of HIV-1 RNA has been reported in the mitochondrial compartment. The rationale for this accumulation is not well understood. Another pertinent question is the origin of HIV-1 RNA with in the mitochondria. These questions need to be addressed meticulously and carefully so that drugs directed to the mitochondria can be designed in order to contain the viral propagation in the mitochondria as well as sustain the mitochondrial integrity. This may significantly influence the ongoing treatment decisions of AIDS patients and aid in better drug designing and consequently improved management and control of AIDS patients.

    Screening of Indigenous medicinal plants for  anti-HIV property: 

 

India harbors a vast repertoire of indigenous medicinal plants. But very few studies have been done on their medicinal property against HIV. Several medicinal plants like Ocimum sp, Withania sp, Bacopa sp., Lithospermum erythrorhizon, Calophyllum lanigerum, Scutellaria sp. and many others have been reported to have anti-HIV activity and we have started optimizing the extraction procedures for the different components of Ocimum and Withania plants for their anti HIV-1 reverse transcriptase activity.  We have cloned the HIV-1 RT gene in pKK vector and expressed in E.coli. The protein has been purified using metal affinity column chromatography.  For the estimation of RT activity primer extension assay will be done in the presence of 3H thymidine. The incorporation of radioactivity will be indicative of RT activity.

 

Teaching

Microbiology, Molecular Biology.Medical Biotechnology, Virology

Awards

Poster Award at ICGEB-IUBMB workshop on Human RNA Viruses at ICGEB, New Delhi 2010.

National Scholarship from Ministry of Human Resource and Development, Government of India, 1990.

Professional Membership

1. Member of American Society of Microbiologist (ASM)

2. Life Member Association of Microbiologists of India

3. Life Member Association for the Promotion of DNA Fingerprinting and other DNA Technologies (ADNAT)

4. Life Member of Indian Science Congress

Important Publications
  1. Virendra N. Pandey, Alok Upadhyay and Binay Chaubey (2009)  Prospects For Antisense Peptide Nucleic Acid (PNA) Therapies For HIV. Expert Opinion On Biological Therapy. 9 (8): 975- 989.

 

  1. Sabyasachi Ganguly, Binay Chaubey, Snehlata Tripathi, Alok Upadhyay, Prasad VSV Neti, Roger W Howell, Virendra Nath Pandey (2008) Pharmacokinetic analysis of Polyamide nucleic acid-cell penetrating peptide conjugates targeted against HIV-1 transactivation response element. Oligonucleotide. 18: 277-286.

 

  1. Binay Chaubey, Snehlata Tripathi, and Virendra N. Pandey (2008) Single Acute-dose and Repeat-doses Toxicity of anti HIV-1 PNA (TAR) - Penetratin Conjugate after Intraperitoneal Administration to Mice. Oligonucleotide. 18(1) :9-20.

 

  1. Binay Chaubey, Snehlata Tripathi, Jérome Désiré, Isabelle Baussanne, Dylan Harris, Jean-Luc Décout and Virendra N. Pandey (2007) Mechanism of RNA Cleavage Catalyzed by Sequence Specific Polyamide Nucleic Acid -Neamine Conjugate. Oligonucleotide. 17(3): 302-13.

 

  1. Snehlata Tripathi, Binay Chaubey, Beverly E. Barton, and Virendra N. Pandey (2007) Anti HIV-1 virucidal activity of polyamide nucleic acid-membrane transducing peptide conjugates targeted to primer binding  site of HIV-1 genome. Virology. 363: 91-103.

 

  1. Harris, D. Zhang, Z. Chaubey, B. Pandey, V. N. (2006)   Identification of cellular factors associated with the 3 nontranslated region of the hepatitis C virus genome. Mol Cell Proteomics. 5 (6): 1006-1018.

 

  1. Snehlata Tripathi, Binay Chaubey, Sabyasachi Ganguly, Dylan Harris, Ralph A Casale and Virendra N.  Pandey (2005) Anti-HIV-1 Activity of Anti-TAR Polyamide Nucleic Acid Conjugated with Various Membrane Transducing Peptides. Nucleic Acids Research. 33 (13):  4345–4356.

 

  1. Binay Chaubey, Snehlata Tripathi, Sabyasachi Ganguly, Dylan Harris,Ralph A. Casale, Virendra N. Pandey (2005) PNA-transportan conjugate targeted to the TAR region of the HIV-1genome exhibits both antiviral and virucidal properties. Virology. 331, 418–428.

 

  1. Emmanuel Riguet, Snehlata Tripathi, Binay Chaubey, Je´roˆme De´sire´, Virendra N. Pandey and Jean-Luc De´cout (2004) A Peptide Nucleic Acid-Neamine Conjugate That Targets and Cleaves HIV-1 TAR RNA Inhibits Viral Replication. J. Med. Chem. 47, 4806-4809.

 

10.   Sarin BC, Chaubey B, Kallan BM and Sehajpal PK (2000) PCR: A tool in detection   of M. tuberculosis. In Environmental Protection. Eds IS Grover, AK Thukral and GS Virk. Scientific Publishers, Jodhpur, India.

 

11.   Dwivedi A, Chaubey B, Sarin BC, Mittar D and Sehajpal PK (2000) A new rapid method  for the isolation of mycobacterial DNA. Ind J Vet Pathol 24: 87-90.

 

 

 

 

Ongoing Research Projects

1. Inhibition of Hepatitis C virus through lentiviral mediated delivery of multiple siRNAs. Approved by Department of Biotechnology (DBT), Ministry of Science and Technology, Govt. of India, in 2008 for 3 years (71.24 Lakh)

2. Mitochondrial dysfunction in response to HIV infection and antiretroviral drug treatment. Approved by Department of Biotechnology (DBT), Ministry of Science and Technology, Govt. of India, in 2007 for 3 years Under Young Scientist Scheme (Rs 29.27 Lakh).

  • 3. Isolation and Indentification of anti-HCV natural compounds from selected    Indian Medicinal Plants.  Approved by Department of Biotechnology (DBT), Ministry of Science and Technology, Govt. of India, in 2013 for 3 years (Rs 35.45 Lakh).
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  • Tie ups and Collaborations
  • Dr. V. N. Pandey, Associate Professor, Department of Biochemistry and Molecular Biology, University of Medicine and Dentistry (UMDNJ), Newark, NJ, USA.
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  • Dr. Sanjay Shukla, Associate Director, Marshfield Research Foundation, Marshfield, Wisconsin, USA.
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  • Prof Banasri Hazra, Research Scientist C (Professor Grade) , UGC, Department of Pharmaceutical Technology, Jadavpur University, Calcutta.
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